Standards Development

Scope

This document describes a real-time PCR procedure for the quantification of the amount of roe deer DNA relative to total mammalian and poultry DNA in meat and meat products [1].

The method has been applied to and is validated for DNA extracted from samples that consist of raw roe deer meat in a raw pork meat background as well as raw and boiled sausages. For quantifying the roe deer content in % (w/w), a reference system based on the myostatin gene was used. The quantification strategy was validated by determining the roe deer content in model meat mixtures containing 2-50 % (w/w) of roe deer meat and pork and a model sausage containing 21 % (w/w) of roe deer meat.

The limit of detection of the roe deer PCR has been determined experimentally to be at least 5 target gene copies or 0.03 % roe deer.

Purpose

For the purposes of this document, the terms and definitions given in ISO 16577 and the following apply.

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