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This document specifies method for determination of gamma oryzanol content in rice bran oil by UV Spectrometry.
This document is applicable to crude and refined rice bran oil.
The multibillion US dollars global rice bran oil market is expected to have a steep growth in coming years owing to the interest of consumers in functional and antioxidant-rich oils for cooking. Rice bran oil is obtained from rice bran, a by-product of rice milling industry and is gaining lot of importance as cooking oil due to its balanced fatty acid profile i.e., mono-unsaturates to poly-unsaturates/saturates and presence of an important micronutrient, gamma oryzanol. It contains several constituents which would potentially provide benefits to health. Unsaponifiable matter in rice bran oil is significantly higher than other edible oils. Unsaponifiable fraction in rice bran oil is rich in antioxidants such as tocopherols and tocotrienols, -oryzanol, phytosterols, polyphenols and squalene. Rice bran oil reduces harmful cholesterol (LDL) without reducing the good cholesterol (HDL), Gamma-Oryzanol (a naturally occurring component in rice bran and rice germ which consists of a mixture of ferulic acid esters of sterols and triterpene alcohols) is the key element responsible for that function apart from its antioxidant properties. Therefore, the oryzanol content serves as a key parameter in assessing the nutritional and therapeutic quality of rice bran oil.
Currently, no ISO standard is available for the determination of gamma oryzanol in rice bran oil. Therefore, it is imperative to develop ISO standard on this subject. The proposed standard is based on the UV Spectrometric method given in Codex standard, CXS 210-1999 ‘Standard for named vegetable oils’ and has been suitably modified for determination of gamma oryzanol in refined rice bran oil in addition to crude rice bran oil. This standard will help not only in evaluating nutritional value of rice bran oil but also in finding out the impact of refining treatment on content of oryzanol in the product. The UV Spectrometric method would provide a simpler, cheaper and faster alternate to the high performance liquid chromatography (HPLC) method and may be used for routine analysis.
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