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ISO/NP 25792 Microbiology of the food chain — detection of hepatitis E virus in meat, liver, meat products and liver products, using real-time RT-PCR

Source:
ISO
Committee:
AW/9 - Microbiology
Categories:
Information management | Standardization. General rules
Comment period start date:
Comment period end date:

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Scope

This document specifies a method for the determination of the presence of HEV from test samples of meat products, or liver and liver products. Following liberation of viruses from the test sample, viral RNA is then extracted by lysis with guanidine thiocyanate and adsorption on silica. Target sequences within the viral RNA are amplified and detected by real-time RT-PCR. The RT-PCR assay can detect multiple genotypes, but it is expected that only zoonotic HEV will be present in the identified matrices. This method has not been validated for detection of HEV in meat.

Purpose

Hepatitis E virus (HEV) can be spread zoonotically through consumption of infected animal products, particularly liver and meat products. The incidence of cases caused by zoonotic HEV infection is rising in several regions. Monitoring of at-risk products for HEV contamination would assist in food safety management. There is, however, no existing standard method for detect ion of HEV in at-risk animal products.

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