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Standards Development
This document is applicable to pure cultures of STEC. The present standard describes the characterization of STEC strains, isolated from any source. In particular, the characterization of STEC strains described here regards:
— Subtyping of the Stx-encoding genes
— Determination of the presence of the eae gene
— Determination of the presence of the aggR gene
— Identification of the presence of genesassociated with the following serogroups: O157, O26, O111, O103, O145, O121 and O45.
The full characterization of the isolated STEC strains is achieved by performing all the modules described here. The characterization scheme is not sequential, and the different modules can be implemented separately based on specific needs (e.g. Regulatory needs, Competent Authority’s requests, clients’ request). Alternative methods may be used, including Whole Genome Sequencing (WGS), provided these are validated according to the most relevant part of reference standard series ISO 16140.
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