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Standards Development
This standard describes the detection and isolation of Shiga toxin-producing Escherichia coli (STEC). The procedure includes the detection by real-time PCR of stx1 and stx2, the major virulence genes of STEC (Reference [1]), in enrichment cultures. Isolation of STEC from the enrichment culture is attempted if one or both stx genes are detected.
This document is applicable to
— products intended for human consumption,
— products intended for animal feeding,
— environmental samples in the area of food and feed production, handling, and
— samples from the primary production stage.
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