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BS EN ISO 13647 ISO 13647 Water quality. Enumeration of culturable microorganisms. Colony count by spread plate inoculation on R2A medium

Source:
ISO
Committee:
EH/3/4 - Microbiological methods
Categories:
Microbiology. Water
Comment period start date:
Comment period end date:
Number of comments:
0

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Scope

This document specifies a method for the enumeration of culturable microorganisms in water by counting the colonies on a low-nutrient agar culture medium by spread plate inoculation after incubation at 22 °C for 7 d.

The method is intended to measure the operational efficiency of the treatment process of public drinking water supplies, including the water in distribution systems and containers. The method is particularly suitable to monitor water for human consumption which is low in nutrients and is distributed in temperatures below 20 °C (Reference [1]). The method can be applied to all types of water, including pool and spa waters.

The low-nutrient agar in use in this document usually gives higher colony counts from water samples than nutrient-rich formulations of culture media typically used for enumeration of culturable microorganisms (References [2], [3], [4]).

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