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This document provides a framework for using in situ underwater optical imaging systems for zooplankton monitoring in marine ecosystem monitoring and assessment. It gives guidance on system requirement and deployment, image acquisition, data processing, taxonomic and size-class identification, quality control, metadata reporting, and data management to improve the consistency and comparability of observations across platforms, regions, and instrument types. This document applies to imaging-based observations of zooplankton, including microzooplankton, mesozooplankton, and macrozooplankton, where technically feasible according to instrument resolution and sampling design. It also addresses key performance considerations such as resolution, detection accuracy, classification, calibration, uncertainty, and system performance under different environmental conditions.
Zooplankton play a critical role in marine ecosystems and the ocean carbon cycle by transferring primary production to higher trophic levels and contributing to biological carbon transport. In situ optical imaging systems now provide non-invasive, high-resolution observations of zooplankton abundance, size structure, distribution, and biomass. However, differences in instrument configuration, deployment practice, image acquisition, taxonomic classification, size measurement, biomass conversion, metadata reporting, and data management have resulted in fragmented and poorly comparable datasets across countries, regions, and observing programs.
This proposed standard will establish a common framework for applying in situ optical zooplankton imaging technologies in marine ecological monitoring and carbon-related assessments. By harmonizing minimum requirements and recommended practices for data acquisition, processing, quality control, performance evaluation, and reporting, the standard will improve comparability, reproducibility, and interoperability among existing and emerging imaging systems. It will support biodiversity monitoring, ecosystem assessment, fisheries management, climate-change studies, ocean carbon accounting, and the development of internationally comparable marine observation datasets. (Please use this field or attach an annex)
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