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Standards Development
This document specifies a flow analysis method with spectrometric detection for the determination of the urea content of milk in mass concentrations ranging from 0 mg/l to 800 mg/l, all in the undiluted sample. Other mass concentration ranges are possible, provided they cover exactly one decade of concentration (e.g. 100 mg/l to 1 000 mg/l). The range of application may be changed by varying the operating conditions.
This kind of determination of urea is used widely in the dairy industry but it is not yet regulated in an official method. The use of the alternative method with pH-difference is running out of instrumental service and has to be replaced by another method such as the proposed NWI. The urea content correlates to the supply of protein in feed of lactating cows and is therefore a marker for farmer to optimize the ration of the herd. Low contents of urea show a ration with less protein. High urea contents indicate a high protein content or a lack of energy. Because of the changing of the ration in short times and therefore e.g. monthly determination of the milk of all cows it is necessary to have a routine method for high throughput of samples. For this reasons a lot of work is done for the validation of the method for the determination of urea with continuous flow analyser.
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