Standards Development
The document investigates the establishment of a method to distinguish the species of origin of herbal medicines in the genetic aspect and to develop a genetic analysis method (DNA barcode). Species identification and classification through DNA analysis is a method of decoding an organism's genes to find similarities and specific parts. Through this, it is possible to 1) confirm the similarity between the species and origins of the herbs, 2) identify the nucleotide sequence that can distinguish the species, and 3) develop a species identification method based on this analysis.
This document could describe a method for the DNA analysis to verify the origin of herbal medicines in order to establish a method for distinguishing the species of origin of herbal medicines. In the case of origin identification using genetic analysis, DNA barcodes could be used. Normally, DNA barcodes use chloroplast and nuclear DNA sites in plants. The identification method is performed through PCR products on an agarose gel or sequencing. The species of origin is discriminated through the combination of 2 or 3 of the selected gene sites. It has been reported that the nucleotide sequence of the barcode region was secured to distinguish the species of each herb’s origin. Therefore, the chloroplast DNA region and nuclear DNA sequencing could be analyzed. This proposal helps build the knowledge database of herbal medicine, and it can be supportive in health care system to incorporate traditional medicinal herbs into main pharmaceuticals by intensive quality control of herbs. Finally, some herbs that could be mixed with similarly related species could also be distinguished.
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