Standards Development
Standardization of procedures to mechanically homogenize aquatic macroinvertebrate specimens collected in the context of, e.g., biodiversity assessments and regulatory biomonitoring of inland surface waters, and subsequent DNA extraction, including terminology, laboratory requirements, contamination avoidance, quality control, recommendation of devices, voucher and DNA storage.
Aquatic macroinvertebrates are the key organismal group required for regulatory biomonitoring of water bodies world-wide (e.g. European Water Framework Directive, 2000/60/EG, South African National Water Act, Australian River Assessment System, National Censuses on River Environments (NCRE) in Japan, US Clean Water Act) and environmental impact assessment. They are also a key group for ecotoxicological assessments as well as in the context of global biodiversity monitoring (e.g. EU biodiversity strategy, Kunming-Montreal Global Biodiversity Framework). With DNA-based approaches, such as DNA metabarcoding, the taxonomic composition of whole aquatic macroinvertebrate communities can be assessed, yet for comparable results standardisation of procedures is essential, including, but not limited to, obtaining a representative subsample for analysis, maximising DNA quality, and avoiding contamination of DNA from other sources.
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