Standards Development
This document specifies a laboratory method for the determination of the absolute and relative fatty acid composition of micro and macro algae by gas chromatography coupled to a flame ionisation detector (GC-FID) of the fatty acid methyl esters.
The standard shall encompass the quantification of the fatty acid composition in algae and algae products, thereby including micro- and macroalgae, according to the definitions adopted by CEN. This quantification shall enable that all fatty acids present at a significant level (> 1 %) in the algal matrix are quantified in an accurate and reproducible way. The concentration of each fatty acid shall be available in relative (in %) and, by means of an appropriate internal standard, absolute (mg/g dw) terms. Moreover, the method described in the standard shall ensure a practical, quick, and safe technical approach, whose protocol details and all related know-how shall be easily and economically transferrable to all the sector stakeholders. The standard shall ensure this objective by a comprehensive and fully detailed description of all technical steps from the sample itself (including its state and form) to the gas chromatographic technique and the calculation of the fatty acid content. The wording shall also avoid any risk of ambiguity or wrong interpretation. Finally, this methodological standard will be informed by other equivalent standards applied to other matrices and will take into account other standards concerning specific treatment or extractive procedure of the sample prior to the fatty acid analysis itself.
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