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Standards Development
This document specifies an extraction method of nanomaterials deposited in organs using the proteinase K.
• The quantification of nanomaterials deposited in organs is the key step for the toxicokinetic studies.
• The lung burden analysis in the inhalation toxicity studies of nanomaterials is required in the revised OECD TG 412 (subacute) and 413 (subchronic).
• The separation of nanomaterials and ionic compounds is important for differentiating nanomaterials from the elements of the body or soluble fraction.
• Chemical or enzymatic digestion methods are commonly used to collect nanomaterials from the organs.
• Chemical digestion methods using acids, alkalis, and oxidants are all common but chemical digestion reagents can also dissolve metal oxides, so, differentiating nanomaterials from the elements of the body or soluble fraction is not possible.
• Chemical digestion methods for carbon-based nanomaterials can damage the structure of the nanomaterials resulting in defects, dissolution and oxidation.
• Meanwhile, enzymatic digestion using proteinase K can be an alternative to chemical lysis, as this approach can discriminate nanomaterials from ionic counterparts and minimize the structural damage of the nanomaterials.
• This method can be one of the methods of PG39 (Lung burden measurement of nanomaterials for inhalation toxicity studies) and toxicokinetic studies of nanomaterials.
• The collected nanomaterials can be quantified by the instrumental analysis based on the physicochemical characteristics of nanomaterials.
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