British Standards Institution

Source:: CEN
Committee:: EH/3/5 - Biological Methods
Categories:: Information management | Standardization. General rules | Environment | Water quality | Water quality. General | Water. Drinking | Water. Sewage | Water. Examination. Biological properties

Scope

This International Standard specifies a method for the determination of the estrogenic activity of water and waste water by means of a reporter gene assay utilizing stably transfected human cells.

This method is applicable to:

- fresh water;

- waste water;

- aqueous extracts and leachates;

- eluates of sediments (fresh water);

- pore water;

- aqueous solutions of single substances or of chemical mixtures;

- drinking water.

Purpose

The estrogenic activity of water and waste water is an relevant endpoint for the quality assessment of (waste)water. The relevance of estrogenic compounds is reflected in the EU-WFD Annex X and the "watch list" as agreed upon by the EU-Commission in August 2013 [2013/39/EU] with obligation to monitor the estrogen receptor agonists EE2 and E2. The here described protocol based on human cells is a robust and highly sensitive bioanalytical method for the determination of estrogenic activity in (waste) water.

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Please email further comments to: debbie.stead@bsigroup.com

Standard timeline

1. Proposal

Proposal start date:

05/01/2021

Proposal end date:

09/02/2021

2. Draft

3. Public Comments

4. Comment Resolution

5. Approval

6. Publication

Learn more about the standards development process

Standards Development

CEN/TC 230 N 1263 , EN ISO 19040-3 Water quality - Determination of the estrogenic potential of water and waste water - Part 3: In vitro human cell-based reporter gene assay

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