Standards Development
This document specifies a method for the determination of the inhibition of root regrowth in duckweeds (Lemna minor) by substances and mixtures contained in water or waste water. This method applies to environmental water samples including treated municipal wastewater and industrial effluents.
The Lemna root regrowth bioassay is a useful test for rapid screening of either wastewater effluents or priority substances spiked into water. This method is complimentary to the longer ISO 20079 Lemna standard.
The new test method differs from the traditional procedures by removing all roots from fronds prior to exposure to toxicants with subsequent measurements on newly developed roots. The root re-growth bioassay also differs from three internationally standardized methods (ISO, OCED and US EPA) in that it is completed in 72 h, the required volume of test solutions is only 3,0 ml and non-axenic plants are used. The new Lemna root method is a simple, rapid, cost-effective, sensitive and precise bioassay to assess the toxic risks of environmental pollutants.
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