British Standards Institution

Source:: ISO
Committee:: LBI/50 - Fine Bubble Technology (FBT)
Categories:: Physics. Chemistry
Comment period start date:: 31/12/2024
Comment period end date:: 23/02/2025
Number of comments:: 0

Comment by:

23rd Feb

Scope

This document describes the procedure for testing and evaluating the generation of ROS such as OH, O2−, H2O2, and O3 in MB and UFB dispersions using the APF assay.

This method is suited for systems generating ROS at physiological levels, not in large quantities, and complements the probe kinetic model for detecting H2O2 in MB and UFB dispersions.

Measurements should be conducted using a quartz cuvette with a 1 cm path length and four transparent sides, in conjunction with fluorescence spectroscopy. As fluorescence values are relative, adherence to this method’s steps is essential, rather than relying on provided calibration lines for direct use.

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Please email further comments to: debbie.stead@bsigroup.com

Standard timeline

1. Proposal (Complete)

Proposal start date:

2. Draft (Complete)

Draft start date:

28/10/2024

3. Public Comments

Public Comments start date:

31/12/2024

Public Comments end date:

23/02/2025

Learn more about the standards development process

Standards Development

BS ISO 24758-2 ISO 24758-2 Fine bubble technology. Evaluation method for determining the reactive oxygen species in ultrafine bubble dispersions. Part 2: APF (3'-(p-aminophenyl) fluorescein) assay

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