British Standards Institution

Source:: ISO
Committee:: EH/4 - Soil quality
Categories:: Soils. Biological properties
Comment period start date:: 15/08/2024
Comment period end date:: 08/10/2024
Number of comments:: 0

Comment by:

8th Oct

Scope

This International Standard specifies the crucial steps of a quantitative real-time polymerase chain reaction (qPCR) method to measure the abundance of selected microbial gene sequences from soil DNA extract which provides an estimation of selected microbial groups.

It is noteworthy that the number of genes is not necessarily directly linked to the number of organisms that are measured. For example, the number of ribosomal operon is ranging from one copy to 20 copies in different bacterial phyla. Therefore, the number of 16S rRNA sequences quantified from soil DNA extracts does not give an exact estimate of the number of soil bacteria. Furthermore, the number of sequences is not necessarily linked to living microorganisms and can comprise sequences amplified from dead microorganisms.

A list of currently well established qPCR Assays to assess selected functional traits of the soil microbiome is listed in Annex C.

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Please email further comments to: debbie.stead@bsigroup.com

Standard timeline

1. Proposal (Complete)

Proposal start date:

2. Draft (Complete)

Draft start date:

24/10/2023

3. Public Comments

Public Comments start date:

15/08/2024

Public Comments end date:

08/10/2024

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Standards Development

BS EN ISO 17601 Soil quality — Estimation of abundance of selected microbial gene sequences by quantitative PCR from DNA directly extracted from soil

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